The optoPlates are a series of open-source devices for high-throughput, multi-color optogenetic experiments in microwell plates. Each well of a 96-well plate can be illuminated with up to 3 wavelengths of light, with independent control of illumination timing and intensity for each wavelength. We designed the optoPlates for robust operation in standard cell culture incubators, and for straightforward assembly and use. The optoPlate-96 can be assembled in a few hours with no specialized equipment, at a total cost of ~$600. See below for full device details, annotated protocols, design files, and code. These devices have enabled us to perform complex, quantitative, and reproducible optogenetic experiments over timescales ranging from minutes to weeks. We hope you will find them equally enabling.
(photo credit: ©Steve Babuljak)
Bugaj LJ and Lim WA. (2019). High throughput multicolor optogenetics in microwell plates. Nature Protocols. doi:10.1038/s41596-019-0178-y link
***please cite this work if you use the optoPlates in a publication
Bugaj, L.J., Sabnis, A.J., Mitchell, A., Garbarino, J.E., Toettcher, J.E., Bivona, T.G., and Lim, W.A. (2018). Cancer mutations and targeted drugs can disrupt dynamic signal encoding by the Ras-Erk pathway. Science 361. link
The optoPlate-96 is, in essence, an LED array formatted to illuminate standard microwell plates. This device allows programmable control of 288 independent channels of light in 96-well format (3 channels/colors per well). Each well position can also illuminate 4-wells of a 384-well plate, and the devices can be readily multiplexed for enhanced throughput. 3D-printed adapters were designed to optically insulate each well from its neighbors. Additionally, an active heatsink minimizes sample heating when light-intensive illumination protocols must be used. The optoPlates were designed primarily for use within cell culture incubators, but can also be adapted for illumination of cells during live-cell microscopy.
96 illumination positions
up to 3 colors per condition. optimized wavelengths for common blue and red mammalian optogenetic probes (blue/red/far-red illumination)
independent control of each color/position
96- or 384-well plate illumination
3D-printed microwell plate adapter and lid
complete optical insulation in each well
designed for long-term incubator experiments
fully integrated, no data cables
powered by Arduino
simple DIY assembly, QC in 3-4 hours
Assembly and use
The optoPlate-96 can be built to illuminate 1, 2 or 3 separate colors per position. Our protocol provides instructions on how to build any of three color combinations that can stimulate the common mammalian cell optogenetic probes:
Blue (LOV2, Cryptochrome2, etc.)
Red/Far-red (Phytochrome B)
Blue/Red/Far-red (for multiplexing 1 and 2)
The optoPlate-96 can be assembled manually following our detailed protocol. Once all necessary components have been ordered/3D printed/laser cut, a fully functional optoPlate-96 can be assembled and tested in 3-4 hours. The design files for PCB printing, 3D-printing, and laser/jet cutting are all provided in our Github repository. To visualize the finished product, explore the interactive 3D models below.
Interactive 3D models
click on an image to interact with the model
Comments? Questions? Bugs?
We would love feedback on the optoPlate-96. What works? What doesn’t? Drop us a note below and we will get back to you as soon as we can.