Papers
Preprints
2024
A temperature-inducible protein module for control of mammalian cell fate.
Benman W#, Huang Z#, Iyengar P, Wilde D, Mumford TR, Bugaj LJ*
bioRxiv Tweetorial
Multiplexed dynamic control of temperature to probe and observe mammalian cells.
Benman W, Iyengar P, Mumford TR, Huang Z, Bugaj LJ*
bioRxiv Tweetorial
2022
Rapid optogenetic clustering in the cytoplasm with BcLOVclust.
Huang, Z, Benman W, Dong L, Bugaj LJ*
Journal of Molecular Biology bioRxiv Tweetorial.
Enhancing single-cell western blotting sensitivity using diffusive analyte blotting and antibody conjugate amplification.
Long MA, Benman W, Petrikas N, Bugaj LJ*, Hughes AJ*
Analytical Chemistry bioRxiv
Optogenetic clustering and membrane translocation of the BcLOV4 photoreceptor.
Pal AA#, Benman W#, Mumford TR, Chow BY, Bugaj LJ*
PNAS bioRxiv Tweetorial
Optogenetic control of YAP reveals a dynamic communication code for stem cell fate and proliferation.
Meyer K, Lammers NC, Bugaj LJ, Garcia HG, Weiner OD*
Nature Communications bioRxiv
Simple detection of submicroscopic protein clusters with a phase-separation-based fluorescent reporter.
Mumford TR, Rae D, Idris A, Gonzalez-Martinez D, Pal AA, Bugaj LJ*
Cell Systems bioRxiv Tweetorial Penn GEN
High-throughput feedback-enabled optogenetic stimulation and spectroscopy in microwell plates.
Benman W#, Datta S#, Gonzalez-Martinez D, Lee G, Hooper J, Qian G, Leavitt G, Salloum L, Ho G, Mhatre S, Magaraci MS, Patterson M, Mannickarottu SG, Malani S, Avalos JL, Chow BY, Bugaj LJ*
Communications Biology bioRxiv Tweetorial
Oncogenic protein condensates modulate growth factor perception and drug tolerance.
Gonzalez-Martinez D#, Roth L#, Mumford TR, Guan J, Huang B, Tulpule A, Bivona TG, Bugaj LJ*
bioRxiv Tweetorial
Cytosolic delivery of small protein scaffolds enables efficient inhibition of Ras and Myc.
Chan A, Wang HH, Haley RM, Song C, Gonzalez-Martinez D, Bugaj LJ, Mitchell MJ*, Tsourkas A*
Molecular Pharmaceutics
Peer-reviewed
2024
2023
2022
Temperature-responsive optogenetic probes of cell signaling
Benman W, Berlew EE, Deng H, Parker C, Kuznetsov IA, Lim B, Siekmann AF, Chow BY, Bugaj LJ*
Nature Chemical Biology Tweetorial Penn News and Views
Vertebrate cells differentially interpret ciliary and extraciliary cAMP
Truong ME, Bilekova S, Choksi SP, Li W, Bugaj LJ, Xu K, Reiter JF*
Cell
T cells selectively filter oscillatory signals on the minutes timescale
O’Donoghue GP, Bugaj LJ, Anderson W, Daniels KG, Rawlings DJ, Lim WA*
PNAS
2021
Single-component optogenetic tools for inducible Rho GTPase signaling
Berlew EE, Kuznetsov, Yamada KK, Bugaj LJ, Boerckel JD, Chow BY*
Advanced Biology
2020
β-catenin Signaling Dynamics Regulate Cell Fate in Differentiating Neural Stem Cells
Rosenbloom AB, Tarczynski M, Lam N, Kane RS*, Bugaj LJ*, Schaffer DV*
PNAS Tweetorial
Reverse and Forward Engineering Multicellular Structures with Optogenetics
Mumford TR, Roth L, Bugaj LJ*
Current Opinion in Biomedical Engineering
Optogenetic control reveals differential promoter interpretation of transcription factor nuclear translocation dynamics.
Chen SY, Osimiri LC, Chevalier MW, Bugaj LJ, Ng AH, Stewart-Ornstein J, Neves LT, El-Samad H*
Cell Systems
Optogenetic Rac1 engineered from membrane lipid-binding RGS-LOV for inducible lamellipodia formation
Berlew EE, Kuznetsov IA, Yamada K., Bugaj LJ, Chow BY*
Photochemical and Photobiological Sciences
High-throughput multicolor optogenetics in microwell plates
Bugaj LJ* and Lim WA
Nature Protocols Tweetorial
2019
2018
Cancer mutations and targeted drugs can disrupt dynamic signal encoding by the Ras/Erk pathway
Bugaj LJ, Sabnis AJ, Mitchell A, Garbarino JE, Toettcher JE*, Bivona TG*, Lim WA*
Science Tweetorial News and Views
2017
Interrogating Cellular Perception and Decision Making with Optogenetic Tools
Bugaj LJ, O’Donoghue GP, Lim WA*
J Cell Biol
2016
Modular engineering of cellular signalling proteins and networks
Gordley RM, Bugaj LJ, Lim WA*
Curr Opin Struct Biol
2015
Regulation of endogenous transmembrane receptors through optogenetic Cry2 clustering
Bugaj LJ, Mesuda CK, Varedi M, Kane RS*, Schaffer DV*
Nature Communications
2014
Age-Associated Increase in BMP Signaling inhibits Hippocampal Neurogenesis
Yousef H, Morgenthaler A, Schlesinger C, Bugaj LJ, Conboy I*, Schaffer DV*
Stem Cells
Systemic attenuation of TGF-β1 pathway in old mice simultaneously rejuvenates neurogenesis and myogenesis by down-modulating MHC I β2 microglobulin.
Yousef H, Conboy M, Morgnethaler A, Schlesinger C, Bugaj LJ, Paliwal P, Greer C, Conboy I*, Schaffer DV*
Oncotarget
2013
Optogenetic protein clustering and signaling activation in mammalian cells
Bugaj LJ, Choksi AT, Mesuda CK, Kane RS*, Schaffer DV*
Nature Methods
Cao J, Arha M, Sudrik C, Bugaj LJ, Schaffer DV, Kane RS*
Light-inducible Activation of Target mRNA Translation in Mammalian Cells
Chem Commun.
2012
Bringing next generation therapeutics to the clinic through synthetic biology
Bugaj LJ and Schaffer DV*
Curr Opin Chem Biol
Upregulation of arginase-II contributes to decreased age-related myocardial contractile reserve
Khan M, Steppan J, Schuleri K, Ryoo S, Tuday E, Bugaj L, Santhanam L, Berkowitz T, Nyhan D, Shoukas AA, Berkowitz DE*
Eur J Appl Physiol
Early changes in vasoreactivity after simulated microgravity are due to an upregulation of the endothelium-dependent nitric oxide/cGMP pathway.
White AR, Ryoo S, Bugaj L, Attarzadeh DO, Thiyagarajan S, Chen K, Attwater S, Abbot B, Li D, Champion HC, Shoukas AA, Nyhan D, Hare JM, Berkowitz DE, Tuday EC*
Eur J Appl Physiol
2010
2009
Arginase Inhibition Restores NOS Coupling and Reverses Endothelial Dysfunction and Vascular Stiffness in Old Rats
Kim JH, Bugaj LJ, Bivalacqua T, Ryoo SS, Oh YJ, Soucy KG, Santhanam L, Camara A, Sikka G, Nyhan D, Shoukas AA, Champion HC, Berkowitz DE*
J Appl Physiol
2006
Arginase modulates myocardial contractility by a NOS-1 dependent mechanism
Steppan J, Ryoo S, Schuleri KH, Gregg C, Hasan RK, White AR, Bugaj LJ, Khan M, Santhanam L, Nyhan D, Shoukas A, Hare J, Berkowitz DE* PNAS